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The first PCIe x16 slot has 16 PCIe lanes and thus can offer the highest throughput compared to the rest of the PCIe slots found on your PC. ...

PCIE x 8 Dual U.2 to PCIe 4.0 X8 Adapter without PCIe Bifurcation Function 0.15 Kilograms PC/Server (PCIe Slot support PCIe Bifurcation: X4X4 or Hyper M.2 X16 mode), Not support Mac Pro.

As a rule of thumb, you should put the graphics card in your motherboard’s first PCIe x16 slot. The first PCIe x16 slot has 16 PCIe lanes and thus can offer the highest throughput compared to the rest of the PCIe slots found on your PC.